Supplementary MaterialsAdditional file 1: Desk S1. cell body and neurites) and in neurites just appearance of SYP (pre-synaptic) and PSD95 (post-synaptic proteins (MAP2 staining was utilized being a marker of neurites), and the amount of overlapping SYP/PSD95 areas (synapses) in the neurites; (B) neurite duration and branch factors per neurite; (C) total BDNF proteins amounts and BDNF appearance ratio, evaluating neurite to cell body. Data are symbolized as mean??S.E.M. of 3C4 natural replicates. Amount S2. Results elicited by Chlorpyrifos (CPF); quantitative evaluation of immunocytochemistry using HCI (Cellomics system). IMR90-produced NSCs had been differentiated for 7 DIV and treated for either 3 or 14?times with 3 different concentrations of CPF (0.37?M, IC20/100, white pubs; 21.01?M, IC5, gray pubs; 37.10?M, IC20, dark bars) compared to solvent control (0.1% DMSO, Ctr) in the respective time point. Analysis of CPF Ponatinib effects on: (A) total (i.e., cell body and neurites) and in neurites only manifestation of SYP (pre-synaptic) and PSD95 (post-synaptic proteins (MAP2 staining was Rabbit Polyclonal to CIB2 used like a marker of neurites), and the number of overlapping SYP/PSD95 places (synapses) in the neurites; (B) neurite size and branch points per neurite; (C) total BDNF protein levels and BDNF manifestation ratio, comparing neurite to cell body. Data are displayed as mean??S.E.M. of 3C4 biological replicates. Number S3. Effects elicited by Lead(II) chloride (Lead); quantitative evaluation of immunocytochemistry using HCI (Cellomics platform). IMR90-derived NSCs were differentiated for 7 DIV and treated for either 3 or 14?days with three different concentrations of Lead (0.0073?M, IC20/100, white bars; 0.17?M, IC5, grey bars; 0.73?M, IC20, black bars) in comparison to solvent control (0.1% DMSO, Ctr) in the respective time point. Analysis of Lead effects on: (A) total (i.e., cell body and neurites) and in neurites only manifestation of SYP (pre-synaptic) and PSD95 (post-synaptic proteins (MAP2 staining was used like a marker of neurites), and the number of overlapping SYP/PSD95 places (synapses) in the neurites; (B) neurite size and branch points per neurite; (C) total BDNF protein levels and BDNF manifestation ratio, comparing neurite to cell body. Data are displayed as Ponatinib mean??S.E.M. of 3C4 biological replicates. Number S4. Effects elicited by Methylmercury(II) chloride (Methyl-Hg); quantitative evaluation of immunocytochemistry using HCI (Cellomics platform). IMR90-derived NSCs were differentiated for 7 DIV and treated for either 3 or 14?days with three different concentrations of Methyl-Hg (0.0013?M, IC20/100, white bars; 0.05?M, IC5, grey Ponatinib bars; 0.13?M, IC20, black bars) in comparison to solvent control (0.1% DMSO, Ctr) in the respective time point. Analysis of Methyl-Hg effects on: (A) total (i.e., cell body and neurites) and in neurites only manifestation of SYP (pre-synaptic) and PSD95 (post-synaptic proteins (MAP2 staining was used being a marker of neurites), and the amount of overlapping SYP/PSD95 areas (synapses) in the neurites; (B) neurite duration and branch factors per neurite; (C) total BDNF proteins amounts and BDNF appearance ratio, evaluating neurite to cell body. Data are symbolized as mean??S.E.M. of 3C4 natural replicates. Amount S5. Results elicited by PCB138; quantitative evaluation of immunocytochemistry using HCI (Cellomics system). IMR90-produced NSCs had been differentiated for 7 DIV and treated for either 3 or 14?times with 3 different concentrations of PCB138 (0.0593?M, IC20/100, white pubs; 3.53?M, IC5, gray pubs; 5.93?M, IC20, dark bars) compared to solvent control (0.1% DMSO, Ctr) on the respective period point. Evaluation of PCB138 results on: (A) total (i.e., cell body and neurites) and Ponatinib in neurites just appearance of SYP (pre-synaptic) and PSD95 (post-synaptic protein (MAP2 staining was.