is an opportunistic pathogen that can cause diarrhea, septicemia, meningitis, and urinary tract infections. Assembler software (Newbler) version 2.9.1, resulting in 14 scaffolds. The “type”:”entrez-protein”,”attrs”:”text”:”P10159″,”term_id”:”54037410″P10159 is definitely 5,080,321?bp, having a G+C content material of 51.7%. Annotation was performed using the Bacterial Annotation System (BASys) (6) and Quick Annotations using Subsystems Technology (RAST) (7) on-line servers and altered by hand. The genome contained 4,768 expected protein-coding sequences (CDSs), 24 rRNAs, 212631-79-3 and 69 tRNAs. In subsystem distribution of the annotation genome, 719 genes were involved in carbohydrate rate of metabolism, 304 genes were involved in protein rate of metabolism, 157 genes were involved in fatty acids, lipids, and isoprenoids, 52 genes were involved in phosphorus rate of metabolism, 119 genes were responsible for virulence, disease, and defense, and 44 genes were associated with phages, prophages, transposable elements, and plasmids. CAV1741 (accession no. “type”:”entrez-nucleotide”,”attrs”:”text”:”CP011657″,”term_id”:”828983113″CP011657), CAV1321 (accession no. “type”:”entrez-nucleotide”,”attrs”:”text”:”CP011612″,”term_id”:”828940051″CP011612), and CFNIH1 (accession no. “type”:”entrez-nucleotide”,”attrs”:”text”:”CP007557″,”term_id”:”619734722″CP007557) were the closest neighbors to strain “type”:”entrez-protein”,”attrs”:”text”:”P10159″,”term_id”:”54037410″P10159, with identities of 96%, 96% and 90%, respectively. The orthologous genes and unique genes among the four genomes were recognized and counted using the Pan-Genomes Analysis Pipeline (PGAP) under the defect parameter (8). Those four genomes shared 3,395 CDSs in total. Strain “type”:”entrez-protein”,”attrs”:”text”:”P10159″,”term_id”:”54037410″P10159 shared 3,613, 3,606, and 3,488 orthologous CDSs with CAV1321, CAV1741, and CFNIH1, respectively. In addition, 787 CDSs from your “type”:”entrez-protein”,”attrs”:”text”:”P10159″,”term_id”:”54037410″P10159 genome were classified as unique, followed by 650 CDSs from CHNIH1, 48 CDSs from CAV1741, and 16 CDSs from CAV1321. To gain a clear understanding of the genomic basis for the observed antibiotic resistance characteristics, the genome was searched for specific genes known to confer antibiotic resistance. The result shows some antibiotic resistance genes 212631-79-3 in the genome conferred resistance against some of the tested antibiotics. Genes, such as strains will allow a better understanding of the resistance mechanisms and aid in restorative agent development in the future. Nucleotide sequence accession number. The complete genome sequence has been deposited in the NCBI database under the accession no. “type”:”entrez-nucleotide”,”attrs”:”text”:”CP012554″,”term_id”:”927043620″CP012554. The version described with this paper is the first version. ACKNOWLEDGMENTS This study was supported by grants from your China Mega-Project on Infectious Disease Prevention (grants 2013ZX10004-605, 2013ZX10004-607, 2013ZX10004-217, and 2011ZX10004-001), the National Hi-Tech Study and Development (863) System of China (grants 2014AA021402, 2012AA022-003, and 2015AA020108), and the National Rabbit Polyclonal to IP3R1 (phospho-Ser1764). Natural Science Basis of China (grant 81572045). Notes This paper was supported by the following give(s): China Mega-Project on Infectious Disease Prevention 2013ZX10004-6052013ZX10004-6072013ZX10004-2172011ZX10004-001 to . National 212631-79-3 Hi-Tech Study and Development (863) System of China 2014AA0214022012AA022-0032015AA020108 to . National Natural Science Basis of China (NSFC) 81572045 to . Footnotes Citation Liu X, Huang Y, Xu X, Zhao Y, Sun Q, Zhang Z, Zhang X, 212631-79-3 Wu Y, Wang J, Zhou D, An X, Pei G, Wang Y, Mi Z, Yin Z, Tong Y. 2016. Total genome sequence of multidrug-resistant strain “type”:”entrez-protein”,”attrs”:”text”:”P10159″,”term_id”:”54037410″P10159, isolated from urine samples from a patient with esophageal carcinoma. Genome Announc 4(1):e01754-15. doi:10.1128/genomeA.01754-15. Recommendations 1. Guerrant RL, Dickens MD, Wenzel RP, Kapikian AZ. 1976. Toxigenic bacterial diarrhea: nursery outbreak including multiple bacterial strains. J Pediatr 89:885C891. doi:10.1016/S0022-3476(76)80591-4. 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