stress W25 was isolated from hydrocarbon-contaminated saline garden soil. and was sequenced using the Illumina HiSeq 2000 system (NORTH PARK, CA). The shotgun sequencing yielded 10,458,934 read pairs (6,863,516 100-bp paired-end reads RHOC with an put in size of 500 bp and 3,595,418 100-bp mate-pair reads with an put in size of 6?kb). Filtered sequencing data had been constructed using SOAPDeNovo v 2.04 (10) and were scaffolded and distance filled up with SSPACE v 2.0 (11) and GapFiller v 1.10 (12). Last assembly contains 18 contigs with an W25 can be phylogenetically linked to BAB-2500 (74.2%) (6), AK1 (71.6%), FJAT-0809-GLX (72.2%) (3), NBRC100599 (72.5%), X23 (72.3%) (4), DSM 25 (66.4%), GI 9 (67.4%) (9), LMG 15441 (67.6%) (5), PE36 (67.6%), buy Nexturastat A phR (69.2%) (8), sp. BC25 (72.2%), sp. CF112 (74.3%) and 423 (72.6%) (7). Seven genes had been identified as involved with hydrocarbon degradation, including 1 alkanal monooxygenase gene and 6 ring-cleavage dioxygenase genes. Furthermore, 7 genes buy Nexturastat A had been defined as involved with suitable solute uptake and synthesis, including 1 ectoine synthase gene, 1 betaine-aldehyde dehydrogenase gene and 5 glycine/betaine ABC transportation genes, which might improve the tolerance to NaCl tension. Information regarding the genome series of W25 will improve our knowledge of microbial hydrocarbon degradation in saline environment. Nucleotide series accession quantity. The draft genome series of W25 continues to be transferred in GenBank beneath the accession quantity “type”:”entrez-nucleotide”,”attrs”:”text”:”AYJU00000000″,”term_id”:”558620586″,”term_text”:”AYJU00000000″AYJU00000000. The edition described with this paper may be the first edition. ACKNOWLEDGMENTS This function was supported from the technology and technology task through the China Country wide Offshore Oil Company (CNOOC-KJ 125 ZDXM 00 000 00 NFCY 2011-04 and CNOOC-KJ 125 ZDXM 25JAbdominal NFCY 2013-01), the technology and technology task of Tianjin Binhai New Region (2012-XJR23017), as well as the postdoctoral creativity fund task of Tianjin. Footnotes Citation Wang X, Jin D, Zhou L, Wu L, An W, Chen Y, Zhao L. 2014. Draft genome series of stress W25, a halotolerant hydrocarbon-degrading bacterium. Genome Announc. 2(1):e01215-13. doi:10.1128/genomeA.01215-13. Sources 1. Mahmoudi N, Porter TM, Zimmerman AR, Fulthorpe RR, Kasozi GN, Silliman BR, Slater GF. 2013. Quick degradation of Deepwater Horizon spilled essential oil by indigenous microbial areas in Louisiana sodium marsh sediments. Environ. Sci. Technol. 47:13303C13312. 10.1021/sera4036072 [PubMed] [Mix Ref] 2. Wang X, Han Z, Bai Z, Tang J, Ma A, He J, Zhuang G. 2011. Archaeal community framework along a gradient of petroleum contaminants in saline-alkali garden soil. J. Environ. Sci. 23:1858C1864. 10.1016/S1001-0742(10)60640-7 [PubMed] [Cross Ref] 3. Che J, Liu B, Lin Y, Tang W, Tang J. 2013. Draft genome series of biocontrol bacterium stress FJAT-0809-GLX. Genome Announc. 1(2):e00160-13. 10.1128/genomeA.00160-13 [PMC free of charge content] [PubMed] [Cross Ref] 4. Chen W, Wang Y, Li D, Li L, Xiao Q, Zhou Q. 2012. Draft genome series of stress X23, a biocontrol agent against bacterial wilt. J. Bacteriol. 194:6634C6635. 10.1128/JB.01312-12 [PMC free of charge content] [PubMed] [Mix Ref] 5. Djukic M, Poehlein A, Thrmer A, Daniel R. 2011. Genome series of LMG 15441, a pathogen of invertebrates. J. Bacteriol. 193:5535C5536. 10.1128/JB.05696-11 [PMC free of charge content] [PubMed] [Mix Ref] 6. Joshi MN, Sharma A, Pandit AS, Pandya RV, Saxena AK, Bagatharia SB. 2013. Draft genome series of sp. stress BAB-2500, a stress that might perform an important part in agriculture. Genome Announc. 1(1):e00021-13. 10.1128/genomeA.00021-13 [PMC free of charge content] [PubMed] [Cross Ref] 7. Yasar Yildiz S, Kambourova M, Arga KY, Toksoy Oner E. 2013. Draft genome series of exopolysaccharide-producing thermophilic bacterium stress 423. Genome Announc. 1(5):e00774-13. 10.1128/genomeA.00774-13 [PMC free of charge content] [PubMed] [Cross Ref] 8. Hugon P, Mishra AK, Nguyen T-T, Raoult D, Fournier P-E. 2013. Non-contiguous completed genome description and sequence of sp. nov. Stand. Genomics Sci. 8:1C14. 10.4056/sigs.3466975 [PMC buy Nexturastat A free article] [PubMed] [Mix Ref] 9. Sharma V, Singh PK, Midha S, Ranjan M, Korpole S, Patil PB. 2012. Genome series of stress GI-9. J. Bacteriol. 194:1279C1279. 10.1128/JB.06659-11 [PMC free of charge article].