reduces severity of acute pancreatitis in animal model Sidhu S, Pandhi P, Malhotra S, Vaiphei K, Khanduja KL in rat model of acute necrotizing pancreatitis. point the animals were sacrificed and blood and pancreatic cells samples were collected. Serum samples were utilized for the evaluation of serum amylase and lipase levels. Pancreatic cells was utilized for the dedication of pancreatic amylase, nucleic acid content and total proteins and rate of DNA synthesis. A part of cells was used for histopathological evaluation. Results: was found to be beneficial in acute pancreatitis. Serum lipase levels were significantly lower in L-arginine group as compared to L-arginine alone group at day 3 and day 14 after pancreatitis but at 24 hrs there was no significant difference between two groups. The serum amylase levels were significantly BMS-265246 lower in L-arginine group as compared to L- arginine alone group at day 14and no significant difference was found at 24 hrs and 3 days after pancreatitis. Nucleic acid content of pancreas was more in L-arginine group as compared to L-arginine alone group at all time points and similarly rate of DNA synthesis was also more in L-arginine group compared to L-arginine alone group. Histopathological examination showed improvement in L-arginine group as compared to L-arginine group. Vitamin C was found to be less efficacious then in all the parameters. Conclusion: was found to be beneficial in acute necrotizing IMPG1 antibody pancreatitis. Treatment with significantly improved the rate of DNA synthesis and total protein content of the pancreas. Histopathological scores were also lower in L-arginine group as compared to L-arginine alone group. 371 Anti-ulcer activity of L flowers in rats Ahmad MP, Alam MS, Ahmad MA, Ahmad S, Najmi AK Jamia Hamdard, New Delhi C 110062, India. The male abortive flowers of Linn. (family Punicaceae) has been used in different single and compound formulations of Unani system of Medicine for the treatment of gastro intestinal disorders. In the present investigation different physicochemical standards of were established and aqueous methanolic extract (AM) of standardized was used for its possible gastro protective activity in Wistar rats against different experimental models. Oral administration of AM (490 and 980 mg kg-1), significantly reduced the ulcer lesion index produced by alcohol (1 ml each), BMS-265246 indomethacin (20 mg kg-1) and aspirin (150 mg kg-1) p.o., dose in rats dependently. The gastro protecting aftereffect of AM was discovered comparable to or even more than that of regular medication (omeprazole 20 mg kg-1, p.o.). Further, in pylorusligated rats AM reduced the ulcer lesions considerably, gastric quantity and total acidity. It had been found out to avoid the ulceration by increasing the mucus and pH secretion in pylorus ligated rats. The initial phytochemical testing of AM exposed the current presence of saponin, flavonoids and tannins, which might be in charge of its gastro protecting impact. HPTLC fingerprints of AM demonstrated existence of 12 peaks at different Rf ideals. The results acquired in present analysis exposed the gastro protecting aftereffect of and requires a organized study upon this traditional treatment. 372 Evolving procedure towards drug advancement from Indian therapeutic vegetation for peptic ulcer disease Palit Gautam Department of Pharmacology, Central Medication Study Institute, Lucknow-226001, India. Peptic Ulcer Disease (PUD), among the main gastrointestinal disorders, takes a targeted restorative strategy. Clinical assessments from the potential anti-ulcer medicines show incidences of relapses, unwanted effects and undesirable drug interaction. To be able to conquer these undesirable results, investigations have already been prolonged for the search of novel molecules from plant sources offering better protection with lower rate of incidences of relapse. Various Indian medicinal plants- have been reported to offer promising anti-ulcer activity. We have directed our endeavors towards establishing the scientific rational in governing the efficacy of natural products in peptic ulcer, using various animal models to explore their anti-ulcer and ulcer healing properties along with their molecular mechanism of action. In this regard we have focused on (OS) which is known to possess various therapeutic properties and identified its potential as an anti-ulcerogenic agent by evaluating against various acute and chronic gastric and duodenal ulcer models in rodents. It significantly reduced free acidity and increased mucin secretion. Moreover, the major gastroprotective effect of OS is mediated through the inhibition of H+ K+-ATPase leading to decrease in acid secretion. Thus with its anti-ulcerogenic and ulcer-healing properties, OS could act as a potent therapeutic agent against BMS-265246 PUD. 373 To study the correlation of apoptosis with severity in caerulein induced pancreatitis Khosla P, Pandhi P, Malhotra S, Nada R1, Rana S2, Bhasin DK1 Postgraduate Institute of Medical Education and Research, Chandigarh, India. Objective: The relationship of apoptosis and pancreatic injury during severe pancreatitis isn’t clear. The purpose of the present.